Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro.

ABSTRACT: OBJECTIVE:The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. METHODS:A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. RESULTS:The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment si

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6946980 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature