Structural and Functional Dissection of the 5' Region of the Notch Gene in Drosophila melanogaster.

ABSTRACT: Notch is a key factor of a signaling cascade which regulates cell differentiation in all multicellular organisms. Numerous investigations have been directed mainly at studying the mechanism of Notch protein action; however, very little is known about the regulation of activity of the gene itself. Here, we provide the results of targeted 5'-end editing of the Drosophila Notch gene in its native environment and genetic and cytological effects of these changes. Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) system in combination with homologous recombination, we obtained a founder fly stock in which a 4-kb fragment, including the 5' nontranscribed region, the first exon, and a part of the first intron of Notch, was r

SUBMITTER: Volkova EI 

PROVIDER: S-EPMC6947440 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature