Global Secretome Characterization of the Pathogenic Yeast <i>Candida glabrata</i>.

ABSTRACT: Secretory proteins are key modulators of host-pathogen interaction. The human opportunistic fungal pathogen Candida glabrata lacks secreted proteolytic activity but possesses 11 glycosylphosphatidylinositol-anchored aspartyl proteases, also referred to as Yapsins (CgYps1-11), that are essential for its virulence. To delineate the role of CgYapsins in interaction with host cells, we have profiled, through liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach, the total secretome of wild-type and Cgyps1-11Δ mutant. The wild-type secretome consisted of 119 proteins which were primarily involved in cell wall organization, carbohydrate metabolism, proteolysis, and translation processes. Of eight CgYapsins identified in the secretome, the release of two m

SUBMITTER: Rasheed M 

PROVIDER: S-EPMC6947636 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature