Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (MacrobrachiumRosenbergii) Using RNA Sequencing.

ABSTRACT: : The giant freshwater prawn (Macrobrachiumrosenbergii) exhibits sex dimorphism between the male and female individuals. To date, the molecular mechanism governing gonadal development was unclear, and limited data were available on the gonad transcriptome of M.rosenbergii. Here, we conducted comprehensive gonadal transcriptomic analysis of female (ZW), super female (WW), and male (ZZ) M.rosenbergii for gene discovery. A total of 70.33 gigabases (Gb) of sequences were generated. There were 115,338 unigenes assembled with a mean size of 1,196 base pair (bp) and N50 of 2,195 bp. Alignment against the National Center for Biotechnology Information (NCBI) non-redundant nucleotide/protein sequence database (NR and NT), the Kyoto Encyclopedia of Gen

SUBMITTER: Jiang J 

PROVIDER: S-EPMC6947849 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature