Comparative proteomic and genomic analyses of Brucella abortus biofilm and planktonic cells.

ABSTRACT: The present study aimed to explore the differences in protein and gene expression of Brucella abortus cultured under biofilm and planktonic conditions. The proteins unique to biofilms and planktonic B. abortus were separated by two‑dimensional (2‑D) electrophoresis and then identified by matrix‑assisted laser desorption/ionization‑tandem time of flight‑mass spectrometry (MALDI‑TOF/TOF‑MS). High‑throughput sequencing and bioinformatic analyses were performed to identify differentially expressed genes between B. abortus cultured under biofilm and planktonic conditions. The proteins and genes identified by proteomic and genomic analyses were further evaluated via western blot and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analyses. 2‑D electrophoresis identified 20

SUBMITTER: Tang T 

PROVIDER: S-EPMC6947884 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature