Increased formation of reactive oxygen species during tumor growth: Ex vivo low-temperature EPR and in vivo bioluminescence analyses.

ABSTRACT: Previous studies have shown that reactive oxygen species (ROS) such as superoxide or hydrogen peroxide generated at low levels can exert a tumor-promoting role via a redox-signaling mechanism. Reports also suggest that both tumorigenesis and tumor growth are associated with enhanced ROS formation. However, whether ROS levels or ROS-derived oxidative marker levels increase during tumor growth remains unknown. In this study, in vivo bioluminescence imaging with a boronate-based pro-luciferin probe was used to assess ROS formation. Additionally, probe-free cryogenic electron paramagnetic resonance was used to quantify a characteristic aconitase [3Fe4S]⁺ center that arises in the tumor tissue of mouse xenografts from the reaction of the native [4Fe4S]²⁺ cluster with super

SUBMITTER: Cheng G 

PROVIDER: S-EPMC6948008 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature