The transferability and evolution of NDM-1 and KPC-2 co-producing Klebsiella pneumoniae from clinical settings.

ABSTRACT:

Background

Emergence of KPC-2 and NDM-1-coproducing carbapenem-resistant Klebsiella pneumoniae (KPC-2-NDM-1-CRKP) has escalated threat of CRKP to healthcare. Currently, only 4 isolates had been reported sporadically. It remains unclear how KPC-2-NDM-1-CRKPs emerged, and whether they are stable and have transmission capacity.

Methods

PCR and Sanger sequencing was used to screen carbapenemase genes for 2057 CRKP isolates, to identify KPC-2-NDM-1-CRKPs. The clinical information of the patients was collected from medical records. Antimicrobial susceptibility, plasmid stability, plasmid conjugation and growth curve were measured. The whole genomes were sequenced on PacBio and Illumina platforms. Similar plasmids were searched against public database. Phylogenetic tree was built b

SUBMITTER: Gao H 

PROVIDER: S-EPMC6948161 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature