Mucosal microbial load in Crohn's disease: A potential predictor of response to faecal microbiota transplantation.

ABSTRACT:

Background

The remission of Crohn's disease (CD) can be accomplished by faecal microbiota transplantation (FMT). However, this procedure has a low success rate, which could be attributed to mis-communication between recipient intestinal mucosa and donor microbiota.

Methods

Here we used a human explant tissue model and an in vivo mouse model to examine changes in recipient intestinal mucosa upon contact with a faecal suspension (FS) obtained from a healthy donor. CD patients provided resected inflamed and non-inflamed mucosal tissues, whereas control colonic mucosa samples were collected from colorectal cancer patients. For the models, mucosal microbiome composition and tissue response were evaluated.

Findings

We show that cytokine release and tissue damage were signif

SUBMITTER: Sarrabayrouse G 

PROVIDER: S-EPMC6948165 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature