Tandem RNA isolation reveals functional rearrangement of RNA-binding proteins on CDKN1B/p27^Kip1 3'UTRs in cisplatin treated cells.

ABSTRACT: Post-transcriptional control of gene expression is mediated via RNA-binding proteins (RBPs) that interact with mRNAs in a combinatorial fashion. While recent global RNA interactome capture experiments expanded the repertoire of cellular RBPs quiet dramatically, little is known about the assembly of RBPs on particular mRNAs; and how these associations change and control the fate of the mRNA in drug-treatment conditions. Here we introduce a novel biochemical approach, termed tobramycin-based tandem RNA isolation procedure (tobTRIP), to quantify proteins associated with the 3'UTRs of cyclin-dependent kinase inhibitor 1B (CDKN1B/p27^Kip1) mRNAs in vivo. P27^Kip1 plays an important role in mediating a cell's response to cisplatin (CP), a widely used chemotherap

SUBMITTER: Iadevaia V 

PROVIDER: S-EPMC6948961 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature