Neuronal-specific microexon splicing of TAF1 mRNA is directly regulated by SRRM4/nSR100.

ABSTRACT: Neuronal microexons represent the most highly conserved class of alternative splicing events and their timed expression shapes neuronal biology, including neuronal commitment and differentiation. The six-nt microexon 34' is included in the neuronal form of TAF1 mRNA, which encodes the largest subunit of the basal transcription factor TFIID. In this study, we investigate the tissue distribution of TAF1-34' mRNA and protein and the mechanism responsible for its neuronal-specific splicing. Using isoform-specific RNA probes and antibodies, we observe that canonical TAF1 and TAF1-34' have different distributions in the brain, which distinguish proliferating from post-mitotic neurons. Knockdown and ectopic expression experiments demonstrate that the neuronal-specific splicing facto

SUBMITTER: Capponi S 

PROVIDER: S-EPMC6948980 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature