Development and Validation of an HPLC Method for the Quantitative Analysis of Bromophenolic Compounds in the Red Alga <i>Vertebrata lanosa</i>.

ABSTRACT: Bromophenols are a class of compounds occurring in red algae that are thought to play a role in chemical protection; however, their exact function is still not fully known. In order to investigate their occurrence, pure standards of seven bromophenols were isolated from a methanolic extract of the epiphytic red alga Vertebrata lanosa collected in Brittany, France. The structures of all compounds were determined by NMR and MS. Among the isolated substances, one new natural product, namely, 2-amino-5-(3-(2,3-dibromo-4,5-dihydroxybenzyl)ureido)pentanoic acid was identified. An HPLC method for the separation of all isolated substances was developed using a Phenomenex C8(2) Luna column and a mobile phase comprising 0.05% trifluoroacetic acid in water and acetonitrile. Method validation s

SUBMITTER: Hofer S 

PROVIDER: S-EPMC6950000 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature