Ammonium Fluoride as Suitable Additive for HILIC-Based LC-HRMS Metabolomics.

ABSTRACT: Hydrophilic Interaction Liquid Chromatography (HILIC) chromatography is widely applied in metabolomics as a complementary strategy to reverse phase chromatography. Nevertheless, it still faces several issues in terms of peak shape and compounds ionization, limiting the automatic de-convolution and data semi-quantification performed through dedicated software. A way to improve the chromatographic and ionization performance of a HILIC method is to modify the electrostatic interactions of the analytes with both mobile and stationary phases. In this study, using a ZIC-HILIC chromatographic phase, we evaluated the performance of ammonium fluoride (AF) as additive salt, comparing its performance to ammonium acetate (AA). Three comparative criteria were selected: (1) identification and peak quali

SUBMITTER: Narduzzi L 

PROVIDER: S-EPMC6950006 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature