Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize.

ABSTRACT: Fumonisin is one of the most prevalent mycotoxins in maize, causing substantial economic losses and potential health risks in human and animals. In the present study, in-house polyclonal IgY antibody against fumonisin group B (FB) was applied for the development of a competitive lateral flow immunoassay detecting these mycotoxins in maize grains with the limit of detection of 4000 µg/kg, which corresponds to the maximum residue limit adopted by The International Codex Alimentarius Commission. To this end, factors affecting the test performance including nitrocellulose membrane type, dilution factor of maize homogenates in running buffer, amount of detection conjugate, and incubation time between detection conjugate and samples were optimized. Under the optimal condition (UniSart ® CN140 ni

SUBMITTER: Tran TV 

PROVIDER: S-EPMC6950345 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature