Microtubules Regulate Localization and Availability of Insulin Granules in Pancreatic Beta Cells.

ABSTRACT: Two key prerequisites for glucose-stimulated insulin secretion (GSIS) in β cells are the proximity of insulin granules to the plasma membrane and their anchoring or docking to the plasma membrane (PM). Although recent evidence has indicated that both of these factors are altered in the context of diabetes, it is unclear what regulates localization of insulin granules and their interactions with the PM within single cells. Here, we demonstrate that microtubule (MT)-motor-mediated transport dynamics have a critical role in regulating both factors. Super-resolution imaging shows that whereas the MT cytoskeleton resembles a random meshwork in the cells' interior, MTs near the cell surface are preferentially aligned with the PM. Computational modeling suggests two consequences of this alignment

SUBMITTER: Bracey KM 

PROVIDER: S-EPMC6950633 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature