A General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology.

ABSTRACT: The electrical membrane potential (V_m) is one of the components of the electrochemical potential of protons across the biological membrane (proton motive force), which powers many vital cellular processes. Because V_m also plays a role in signal transduction, measuring it is of great interest. Over the years, a variety of techniques have been developed for the purpose. In bacteria, given their small size, Nernstian membrane voltage probes are arguably the favorite strategy, and their cytoplasmic accumulation depends on V_m according to the Nernst equation. However, a careful calibration of Nernstian probes that takes into account the tradeoffs between the ease with which the signal from the dye is observed and the dyes' interactions with cellular physiology i

SUBMITTER: Mancini L 

PROVIDER: S-EPMC6950638 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature