Role of Membrane Potential on Entry of Cell-Penetrating Peptide Transportan 10 into Single Vesicles.

ABSTRACT: Cell-penetrating peptides (CPPs) can translocate across plasma membranes to enter the cytosol of eukaryotic cells without decreasing cell viability. We revealed the mechanism underlying this translocation by examining the effect of membrane potential, φ_m, on the entry of a CPP, transportan 10 (TP10), into the lumen of single giant unilamellar vesicles (GUVs). For this purpose, we used the single GUV method to detect the entry of carboxyfluorescein (CF)-labeled TP10 (CF-TP10) into the lumen of single GUVs. First, we used various K⁺ concentration differences to apply different negative membrane potentials on single GUVs containing gramicidin A in their membrane and confirmed these potentials using the φ_m-sensitive fluorescent probe 3,3'-dihexyloxacarbocyanine

SUBMITTER: Moghal MMR 

PROVIDER: S-EPMC6950768 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature