CNCC: an analysis tool to determine genome-wide DNA break end structure at single-nucleotide resolution.

ABSTRACT:

Background

DNA double-stranded breaks (DSBs) are potentially deleterious events in a cell. The end structures (blunt, 3'- and 5'-overhangs) at DSB sites contribute to the fate of their repair and provide critical information concerning the consequences of the damage. Therefore, there has been a recent eruption of DNA break mapping and sequencing methods that aim to map at single-nucleotide resolution where breaks are generated genome-wide. These methods provide high resolution data for the location of DSBs, which can encode the type of end-structure present at these breaks. However, genome-wide analysis of the resulting end structures has not been investigated following these sequencing methods.

Results

To address this analysis gap, we develop the use of a coverage-normalize

SUBMITTER: Szlachta K 

PROVIDER: S-EPMC6950916 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature