Neuropeptide Y regulates proliferation and apoptosis in granulosa cells in a follicular stage-dependent manner.

ABSTRACT: BACKGROUND:The complex regulatory mechanism involved in ovarian follicular development is not completely understood. Neuronal neuropeptide Y (NPY) is involved in the regulation of feeding behavior, energy homeostasis, and reproduction behavior, while its function in ovarian follicular development is not clear. The objective of this study was to investigate if and how NPY regulates follicle development in the ovary. METHODS:All experiments were performed using Sprague Dawley rats. To understand NPY expression pattern at different stages of follicular development, NPY content was assessed using immunohistochemistry in individual follicles. NPY and its receptors expression pattern were evaluated in granulosa cells isolated from preantral (PA), early antral (EA) and late antral follicles (LAF)

SUBMITTER: Urata Y 

PROVIDER: S-EPMC6950994 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature