MiR-181c Activates Mitochondrial Calcium Uptake by Regulating MICU1 in the Heart.

ABSTRACT: Background Translocation of miR-181c into cardiac mitochondria downregulates the mitochondrial gene, mt-COX1. miR-181c/d^-/- hearts experience less oxidative stress during ischemia/reperfusion (I/R) and are protected against I/R injury. Additionally, miR-181c overexpression can increase mitochondrial matrix Ca²⁺ ([Ca²⁺]_m), but the mechanism by which miR-181c regulates [Ca²⁺]_m is unknown. Methods and Results By RNA sequencing and analysis, here we show that hearts from miR-181c/d^-/- mice overexpress nuclear-encoded Ca²⁺ regulatory and metabolic pathway genes, suggesting that alterations in miR-181c and mt-COX1 perturb mitochondria-to-nucleus retrograde signaling and [Ca²⁺]_m regulatio

SUBMITTER: Banavath HN 

PROVIDER: S-EPMC6951067 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature