Coexpression of CD163 and CD141 identifies human circulating IL-10-producing dendritic cells (DC-10).

ABSTRACT: Tolerogenic dendritic cells (DCs) are key players in maintaining immunological homeostasis, dampening immune responses, and promoting tolerance. DC-10, a tolerogenic population of human IL-10-producing DCs characterized by the expression of HLA-G and ILT4, play a pivotal role in promoting tolerance via T regulatory type 1 (Tr1) cells. Thus far, the absence of markers that uniquely identify DC-10 has limited in vivo studies. By in vitro gene expression profiling of differentiated human DCs, we identified CD141 and CD163 as surface markers for DC-10. The coexpression of CD141 and CD163 in combination with CD14 and CD16 enables the ex vivo isolation of DC-10 from the peripheral blood. CD14⁺CD16⁺CD141⁺CD163⁺ cells isolated from the peripheral blood o

SUBMITTER: Comi M 

PROVIDER: S-EPMC6952411 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature