Separating distinct structures of multiple macromolecular assemblies from cryo-EM projections.

ABSTRACT: Single particle analysis for structure determination in cryo-electron microscopy is traditionally applied to samples purified to near homogeneity as current reconstruction algorithms are not designed to handle heterogeneous mixtures of structures from many distinct macromolecular complexes. We extend on long established methods and demonstrate that relating two-dimensional projection images by their common lines in a graphical framework is sufficient for partitioning distinct protein and multiprotein complexes within the same data set. The feasibility of this approach is first demonstrated on a large set of synthetic reprojections from 35 unique macromolecular structures spanning a mass range of hundreds to thousands of kilodaltons. We then apply our algorithm on cryo-EM data collected fro

SUBMITTER: Verbeke EJ 

PROVIDER: S-EPMC6952565 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature