A Software Tool for High-Throughput Real-Time Measurement of Intensity-Based Ratio-Metric FRET.

ABSTRACT: Förster resonance energy transfer (FRET) is increasingly used for non-invasive measurement of fluorescently tagged molecules in live cells. In this study, we have developed a freely available software tool MultiFRET, which, together with the use of a motorised microscope stage, allows multiple single cells to be studied in one experiment. MultiFRET is a Java plugin for Micro-Manager software, which provides real-time calculations of ratio-metric signals during acquisition and can simultaneously record from multiple cells in the same experiment. It can also make other custom-determined live calculations that can be easily exported to Excel at the end of the experiment. It is flexible and can work with multiple spectral acquisition channels. We validated this software by comparing the output

SUBMITTER: Ramuz M 

PROVIDER: S-EPMC6952787 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature