A New Microfluidic Platform for Studying Natural Killer Cell and Dendritic Cell Interactions.

ABSTRACT: The importance of the bi-directional natural killer-dendritic cell crosstalk in coordinating anti-tumour and anti-microbial responses in vivo has been well established. However, physical parameters associated with natural killer-dendritic cell interactions have not been fully elucidated. We have previously used a simple "Y" shaped microfluidic device to study natural killer cell-migratory responses toward chemical gradients from a conditioned medium of dendritic cells. There are, however, limitations of the Y-shaped microfluidic devices that could not support higher throughput analyses and studies of cell-cell interactions. Here, we report two novel microfluidic devices (D3-Chip, T2-Chip) we applied in advanced studies of natural killer-cell migrations and their interactions with dendritic

SUBMITTER: Hipolito J 

PROVIDER: S-EPMC6952968 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature