Post-ER Stress Biogenesis of Golgi Is Governed by Giantin.

ABSTRACT:

Background

The Golgi apparatus undergoes disorganization in response to stress, but it is able to restore compact and perinuclear structure under recovery. This self-organization mechanism is significant for cellular homeostasis, but remains mostly elusive, as does the role of giantin, the largest Golgi matrix dimeric protein.

Methods

In HeLa and different prostate cancer cells, we used the model of cellular stress induced by Brefeldin A (BFA). The conformational structure of giantin was assessed by proximity ligation assay and atomic force microscopy. The post-BFA distribution of Golgi resident enzymes was examined by 3D SIM high-resolution microscopy.

Results

We detected that giantin is rather flexible than an extended coiled-coil dimer and BFA-induced Golgi disasse

SUBMITTER: Frisbie CP 

PROVIDER: S-EPMC6953117 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature