A high-throughput BAC end analysis protocol (BAC-anchor) for profiling genome assembly and physical mapping.

ABSTRACT: Traditional approaches for sequencing insertion ends of bacterial artificial chromosome (BAC) libraries are laborious and expensive, which are currently some of the bottlenecks limiting a better understanding of the genomic features of auto- or allopolyploid species. Here, we developed a highly efficient and low-cost BAC end analysis protocol, named BAC-anchor, to identify paired-end reads containing large internal gaps. Our approach mainly focused on the identification of high-throughput sequencing reads carrying restriction enzyme cutting sites and searching for large internal gaps based on the mapping locations of both ends of the reads. We sequenced and analysed eight libraries containing over 3 200 000 BAC end clones derived from the BAC library of the tetraploid potato cultivar C88 d

SUBMITTER: Yang X 

PROVIDER: S-EPMC6953197 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature