Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma.

ABSTRACT:

Background

Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technology (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in different endemic countries.

Results

The qSAT was specific for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml

SUBMITTER: Martianez-Vendrell X 

PROVIDER: S-EPMC6953214 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature