Utilizing field collected insects for next generation sequencing: Effects of sampling, storage, and DNA extraction methods.

ABSTRACT: DNA sequencing technologies continue to advance the biological sciences, expanding opportunities for genomic studies of non-model organisms for basic and applied questions. Despite these opportunities, many next generation sequencing protocols have been developed assuming a substantial quantity of high molecular weight DNA (>100 ng), which can be difficult to obtain for many study systems. In particular, the ability to sequence field-collected specimens that exhibit varying levels of DNA degradation remains largely unexplored. In this study we investigate the influence of five traditional insect capture and curation methods on Double-Digest Restriction Enzyme Associated DNA (ddRAD) sequencing success for three wild bee species. We sequenced a total of 105 specimens (between 7-13 specimens

SUBMITTER: Ballare KM 

PROVIDER: S-EPMC6953651 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature