Common alleles of CMT2 and NRPE1 are major determinants of CHH methylation variation in Arabidopsis thaliana.

ABSTRACT: DNA cytosine methylation is an epigenetic mark associated with silencing of transposable elements (TEs) and heterochromatin formation. In plants, it occurs in three sequence contexts: CG, CHG, and CHH (where H is A, T, or C). The latter does not allow direct inheritance of methylation during DNA replication due to lack of symmetry, and methylation must therefore be re-established every cell generation. Genome-wide association studies (GWAS) have previously shown that CMT2 and NRPE1 are major determinants of genome-wide patterns of TE CHH methylation. Here we instead focus on CHH methylation of individual TEs and TE-families, allowing us to identify the pathways involved in CHH methylation simply from natural variation and confirm the associations by comparing them with mutant phenotypes. M

SUBMITTER: Sasaki E 

PROVIDER: S-EPMC6953882 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature