Extreme Nonuniform Sampling for Protein NMR Dynamics Studies in Minimal Time.

ABSTRACT: NMR spectroscopy is an extraordinarily rich source of quantitative dynamics of proteins in solution using spin relaxation or chemical exchange saturation transfer (CEST) experiments. However, ¹⁵N-CEST measurements require prolonged multidimensional, so-called pseudo-3D HSQC experiments where the pseudo dimension is a radio frequency offset Δω of a weak ¹⁵N saturation field. Nonuniform sampling (NUS) approaches have the potential to significantly speed up these measurements, but they also carry the risk of introducing serious artifacts and the systematic optimization of nonuniform sampling schedules has remained elusive. It is demonstrated here how this challenge can be addressed by using fitted cross-peaks of a reference 2D HSQC experiment as footprints, which are sub

SUBMITTER: Jameson G 

PROVIDER: S-EPMC6953986 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature