Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins.

ABSTRACT: Pyoverdine (PVDI) has been reported to act both as a siderophore for scavenging iron (a key nutrient) and a signaling molecule for the expression of virulence factors. This compound is itself part of a core set of virulence factors produced by Pseudomonas aeruginosa during infections. Once secreted into the bacterial environment and having scavenged ferric iron, PVDI-Fe³⁺ is taken back into the P. aeruginosa periplasm via the outer membrane transporters FpvAI and FpvB. Iron release from PVDI in the bacterial periplasm involves numerous proteins encoded by the fpvGHJKCDEF genes and a mechanism of iron reduction. Here, we investigated the global interacting network between these various proteins using systematic bacterial two-hybrid screening. We deciphered a network of five inter

SUBMITTER: Bonneau A 

PROVIDER: S-EPMC6954188 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature