Structure of an RNA aptamer in complex with the fluorophore tetramethylrhodamine.

ABSTRACT: RNA aptamers-artificially created RNAs with high affinity and selectivity for their target ligand generated from random sequence pools-are versatile tools in the fields of biotechnology and medicine. On a more fundamental level, they also further our general understanding of RNA-ligand interactions e. g. in regard to the relationship between structural complexity and ligand affinity and specificity, RNA structure and RNA folding. Detailed structural knowledge on a wide range of aptamer-ligand complexes is required to further our understanding of RNA-ligand interactions. Here, we present the atomic resolution structure of an RNA-aptamer binding to the fluorescent xanthene dye tetramethylrhodamine. The high resolution structure, solved by NMR-spectroscopy in solution, reveals binding feature

SUBMITTER: Duchardt-Ferner E 

PROVIDER: S-EPMC6954400 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature