Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters.

ABSTRACT:

Background

Transposable elements (TEs) are a diverse group of self-mobilizing DNA elements. Transposition has been exploited as a powerful tool for molecular biology and genomics. However, transposition is sometimes limited because of auto-regulatory mechanisms that presumably allow them to cohabit within their hosts without causing excessive genomic damage. The papillation assay provides a powerful visual screen for hyperactive transposases. Transposition is revealed by the activation of a promoter-less lacZ gene when the transposon integrates into a non-essential gene on the host chromosome. Transposition events are detected as small blue speckles, or papillae, on the white background of the main Escherichia coli colony.

Results

We analysed the parameters of

SUBMITTER: Tellier M 

PROVIDER: S-EPMC6954556 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature