DECENT: differential expression with capture efficiency adjustmeNT for single-cell RNA-seq data.

ABSTRACT:

Motivation

Dropout is a common phenomenon in single-cell RNA-seq (scRNA-seq) data, and when left unaddressed it affects the validity of the statistical analyses. Despite this, few current methods for differential expression (DE) analysis of scRNA-seq data explicitly model the process that gives rise to the dropout events. We develop DECENT, a method for DE analysis of scRNA-seq data that explicitly and accurately models the molecule capture process in scRNA-seq experiments.

Results

We show that DECENT demonstrates improved DE performance over existing DE methods that do not explicitly model dropout. This improvement is consistently observed across several public scRNA-seq datasets generated using different technological platforms. The gain in improvement is especially large

SUBMITTER: Ye C 

PROVIDER: S-EPMC6954660 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature