Dataset Information

Validity and reliability evidence of a point of care assessment of salivary cortisol and ?-amylase: a pre-registered study.

ABSTRACT: Purpose:The iPro Cube is a small portable point-of-care device designed to analyse salivary markers of stress in a user-friendly manner (e.g., fast, convenient). Our aim was to test the reliability and validity of the iPro Cube to measure salivary cortisol and ?-amylase as compared to the common laboratory standard method (ELISA immunoassay) prior to and after moderate intensity exercise. Methods:The study was a repeated measures, pre-registered design, and statistical framework that incorporated prior knowledge directly into the estimation process. Twenty-nine individuals (age = 27.4 ± 6.6 y; body-mass = 70.8 ± 11.3 kg; height = 1.74 ± 0.92 m; 18 males) completed a single PWC75%HRmax, with repeated measures of salivary cortisol and -amylase pre, immediately post, and 30 min post-exercise. Results:Correlation between the iPro Cube and laboratory-based assessments of salivary cortisol was moderate-to-large (0.53 > r < 0.81) across all three testing points. In contrast, correlation between the iPro Cube and laboratory-based assessments of -amylase was small-to-moderate (0.25 > r < 0.46). We found a large correlation between duplicate samples of iPro Cube cortisol assessment (0.75 > r < 0.82), and a moderate-to-large correlation for -amylase (0.51> r < 0.77). Conclusions:The iPro Cube is capable of taking measures of salivary cortisol that are moderately correlated to values obtained via ELISA immunoassay, however the unit underestimates salivary cortisol and overestimates salivary -amylase at rest and post-moderate intensity exercise. It is recommended that researchers continue using standard laboratory techniques to assess these salivary stress markers.

SUBMITTER: Ducker KJ

PROVIDER: S-EPMC6954686 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Validity and reliability evidence of a point of care assessment of salivary cortisol and <i>α</i>-amylase: a pre-registered study.

Ducker Kagan J KJ Lines Robin L J RLJ Chapman Michael T MT Peeling Peter P McKay Alannah K A AKA Gucciardi Daniel F DF

PeerJ 20200108

<h4>Purpose</h4>The iPro Cube is a small portable point-of-care device designed to analyse salivary markers of stress in a user-friendly manner (e.g., fast, convenient). Our aim was to test the reliability and validity of the iPro Cube to measure salivary cortisol and <i>α</i>-amylase as compared to the common laboratory standard method (ELISA immunoassay) prior to and after moderate intensity exercise.<h4>Methods</h4>The study was a repeated measures, pre-registered design, and statistical fram ...[more]

PMID: 31938581

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Project description:BackgroundThe use of salivary biomarkers has garnered attention because the composition of saliva reflects the body's physiological state. Saliva contains a wide range of components, including peptides, nucleic acids, electrolytes, enzymes, and hormones. It has been reported that salivary alpha-amylase and cortisol are biomarkers of stress related biomarker in diseased dogs; however, evaluation of salivary alpha-amylase and cortisol pre- and post- operation has not been studied yet. The aim of this study was to evaluate salivary alpha-amylase and cortisol levels in dogs before and after they underwent surgery and investigate the association between the salivary alpha-amylase and cortisol activity and pain intensity. For this purpose, a total of 35 dogs with disease-related pain undergoing orthopedic and soft tissue surgeries were recruited. Alpha-amylase and cortisol levels in the dogs' saliva and serum were measured for each using a commercially available canine-specific enzyme-linked immunosorbent assay kit, and physical examinations (measurement of heart rate and blood pressure) were performed. In addition, the dogs' pre- and post-operative pain scores determined using the short form of the Glasgow Composite Measure Pain Scale (CMPS-SF) were evaluated.ResultsAfter surgery, there was a significant decrease in the dogs' pain scores (0.4-fold for the CMPS-SF, p < 0.001) and serum cortisol levels (0.73-fold, p < 0.01). Based on their pre-operative CMPS-SF scores, the dogs were included in either a high-pain-score group or a low-pain-score group. After the dogs in the high-pain-score group underwent surgical intervention, there was a significant decrease in their CMPS-SF scores and levels of salivary alpha-amylase, serum alpha-amylase, and serum cortisol. Additionally, there was a positive correlation between salivary alpha-amylase levels and CMPS-SF scores in both the high- and low-pain-score groups.ConclusionsThe measurement of salivary alpha amylase can be considered an important non-invasive tool for the evaluation of pain-related stress in dogs.

Project description:IntroductionAcademic stress is a prevalent problem among university students, affecting both their psychological well-being and academic performance. This study aims to investigate the mediating roles of biological and psycho-behavioural variables in the relationship between academic stress and academic performance over the course of a semester. Through a longitudinal approach and using accessible data collection technologies, the results will enable the design of effective interventions to mitigate the impact of academic stress.Hypotheses(i) Biological variables related to academic performance will mediate the relationship between academic stress and students' academic performance. (ii) Psycho-behavioural variables will also act as mediators in this relationship, impacting academic performance differently.General objectiveTo explore the mediating roles of biological and psycho-behavioural variables in the relationship between academic stress and academic performance over the course of a university semester.DesignA longitudinal non-experimental observational design will be applied. Data will be collected in three assessment cycles, each consisting of three consecutive weeks during the academic semester.ParticipantsA sample of 160 undergraduate students from the Faculty of Medicine of the University of Concepción will be included. Students will be recruited on a voluntary basis through social networks and student associations. Students under psychological or pharmacological treatment will also be included to more representatively reflect the student reality and to ensure the ecological validity of the study.Biological and psycho-behavioural data collectionParticipants will answer electronic questionnaires on academic stress and psycho-behavioural variables three times a week via the REDCap platform. In addition, smart devices will be used to continuously collect biological data such as heart rate, oxygen saturation, and sleep patterns. Students will also collect saliva samples three times a week to measure cortisol levels, and alpha-amylase enzyme activity.Statistical analysis(i) Descriptive analysis of variables will be performed using measures of central tendency and dispersion for continuous variables and frequencies and percentages for categorical variables. (ii) Bivariate and multivariate analyses will be conducted to compare groups. (iii) Random intercept cross-lagged models will be used to assess the direction and reciprocal effects between variables over time. To analyze mediations, structural models (SEM) will be applied, considering biological and psycho-behavioural variables as mediators.Expected resultsIt is anticipated that (i) biological variables, such as cortisol and salivary alpha-amylase, will play a significant mediating role in the relationship between academic stress and academic performance, particularly towards the end of the semester. (ii) psycho-behavioural variables will also have a mediating effect, with different impacts on academic performance depending on the level of stress experienced. The use of accessible technologies and non-invasive methods such as saliva sample collection will provide a replicable model for future research.

Project description:BackgroundCortisol is an essential hormone in the regulation of the stress response along the HPA axis, and salivary cortisol has been used as a measure of free circulating cortisol levels. Recently, salivary alpha-amylase (sAA) has also emerged as a novel biomarker for psychosocial stress responsiveness within the sympathetic adrenomedullary (SAM) system.Principal findingsWe measured sAA and salivary cortisol in healthy volunteers after exposure to the Trier Social Stress Test (TSST) and electric stimulation stress. One hundred forty-nine healthy volunteers participated in this study. All subjects were exposed to both the TSST and electric stimulation stress on separate days. We measured sAA and salivary cortisol levels three times immediately before, immediately after, and 20 min after the stress challenge. The State (STAI-S) and Trait (STAI-T) versions of the Spielberger Anxiety Inventory test and the Profile of Mood State (POMS) tests were administered to participants before the electrical stimulation and TSST protocols. We also measured HF, LF and LF/HF Heart Rate Variability ratio immediately after electrical stimulation and TSST exposure. Following TSST exposure or electrical stimulation, sAA levels displayed a rapid increase and recovery, returning to baseline levels 20 min after the stress challenge. Salivary cortisol responses showed a delayed increase, which remained significantly elevated from baseline levels 20 min after the stress challenge. Analyses revealed no differences between men and women with regard to their sAA response to the challenges (TSST or electric stimulations), while we found significantly higher salivary cortisol responses to the TSST in females. We also found that younger subjects tended to display higher sAA activity. Salivary cortisol levels were significantly correlated with the strength of the applied electrical stimulation.ConclusionsThese preliminary results suggest that the HPA axis (but not the SAM system) may show differential response patterns to distinct kinds of stressors.

Dataset Information

Validity and reliability evidence of a point of care assessment of salivary cortisol and ?-amylase: a pre-registered study.

Publications

Validity and reliability evidence of a point of care assessment of salivary cortisol and <i>α</i>-amylase: a pre-registered study.

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