Genes of the cGMP-PKG-Ca²⁺ signaling pathway are alternatively spliced in cardiomyopathy: Role of RBFOX2.

ABSTRACT: Aberrations in the cGMP-PKG-Ca²⁺ pathway are implicated in cardiovascular complications of diverse etiologies, though involved molecular mechanisms are not understood. We performed RNA-Seq analysis to profile global changes in gene expression and exon splicing in Chagas disease (ChD) murine myocardium. Ingenuity-Pathway-Analysis of transcriptome dataset identified 26 differentially expressed genes associated with increased mobilization and cellular levels of Ca²⁺ in ChD hearts. Mixture-of-isoforms and Enrichr KEGG pathway analyses of the RNA-Seq datasets from ChD (this study) and diabetic (previous study) murine hearts identified alternative splicing (AS) in eleven genes (Arhgef10, Atp2b1, Atp2a3, Cacna1c, Itpr1, Mef2a, Mef2d, Pde2a, Plcb1, Plcb4, and Ppp1r12a) of the

SUBMITTER: Wan X 

PROVIDER: S-EPMC6954967 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature