Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy.

ABSTRACT: Integration of dual-barrel membrane patch-ion channel probes (MP-ICPs) to scanning ion conductance microscopy (SICM) holds promise of providing a revolutionized approach of spatially resolved chemical sensing. A series of experiments are performed to further the understanding of the system and to answer some fundamental questions, in preparation for future developments of this approach. First, MP-ICPs are constructed that contain different types of ion channels including transient receptor potential vanilloid 1 and large conductance Ca2⁺ -activated K⁺ channels to establish the generalizability of the methods. Next, the capability of the MP-ICP platforms in single ion channel activity measurements is proved. In addition, the interplay between the SICM barrel and the IC

SUBMITTER: Shi W 

PROVIDER: S-EPMC6955111 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature