Visualisation of ribosomes in <i>Drosophila</i> axons using Ribo-BiFC.

ABSTRACT: The distribution of assembled, and potentially translating, ribosomes within cells can be visualised in Drosophila by using Bimolecular Fluorescence Complementation (BiFC) to monitor the interaction between tagged pairs of 40S and 60S ribosomal proteins (RPs) that are close neighbours across inter-subunit junctions in the assembled 80S ribosome. Here we describe transgenes expressing two novel RP pairs tagged with Venus-based BiFC fragments that considerably increase the sensitivity of this technique we termed Ribo-BiFC. This improved method should provide a convenient way of monitoring the local distribution of ribosomes in most Drosophila cells and we suggest that it could be implemented in other organisms. We visualised 80S ribosomes in different neurons, particularly phot

SUBMITTER: Singh AK 

PROVIDER: S-EPMC6955225 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature