Shear force sensing of epithelial Na⁺ channel (ENaC) relies on N-glycosylated asparagines in the palm and knuckle domains of αENaC.

ABSTRACT: Mechanosensitive ion channels are crucial for normal cell function and facilitate physiological function, such as blood pressure regulation. So far little is known about the molecular mechanisms of how channels sense mechanical force. Canonical vertebrate epithelial Na⁺ channel (ENaC) formed by α-, β-, and γ-subunits is a shear force (SF) sensor and a member of the ENaC/degenerin protein family. ENaC activity in epithelial cells contributes to electrolyte/fluid-homeostasis and blood pressure regulation. Furthermore, ENaC in endothelial cells mediates vascular responsiveness to regulate blood pressure. Here, we provide evidence that ENaC's ability to mediate SF responsiveness relies on the "force-from-filament" principle involving extracellular tethers and the extracellular matri

SUBMITTER: Knoepp F 

PROVIDER: S-EPMC6955349 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature