Localization of ATP-sensitive K+ channel subunits in rat liver.

ABSTRACT: BACKGROUND:ATP-sensitive K+ (KATP) channels were originally found in cardiac myocytes by Noma in 1983. KATP channels were formed by potassium ion-passing pore-forming subunits (Kir6.1, Kir6.2) and regulatory subunits SUR1, SU2A and SUR2B. A number of cells and tissues have been revealed to contain these channels including hepatocytes, but detailed localization of these subunits in different types of liver cells was still uncertain. AIM:To investigate the expression of KATP channel subunits in rat liver and their localization in different cells of the liver. METHODS:Rabbit anti-rat SUR1 peptide antibody was raised and purified by antigen immunoaffinity column chromatography. Four of Sprague-Dawley rats were used for liver protein extraction for immunoblot analysis, seven of them were used f

SUBMITTER: Zhou M 

PROVIDER: S-EPMC6955576 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature