SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding.

ABSTRACT:

Background

Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX). In SELEX, monitoring the enriching statuses of aptamer candidates during the process is a key step until today. Conformational change of an aptamer caused by target-binding in gel can be used to indicate its statuses of binding.

Results

In this study, an easy-to-implement gel-based diffusion method (GBDM) was developed to monitor the interaction between enriched aptamer candidates and their targets. In order to prove the concept, characterization of aptamers targeting their targets including protein (thrombin) and non-protein molecules (acetamiprid, ATP, atrazine, profenofos and

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6956507 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature