ROCK2 inhibition enhances the thermogenic program in white and brown fat tissue in mice.

ABSTRACT: The RhoA/ROCK-mediated actin cytoskeleton dynamics have been implicated in adipogenesis. The two ROCK isoforms, ROCK1 and ROCK2, are highly homologous. The contribution of ROCK2 to adipogenesis in vivo has not been elucidated. The present study aimed at the in vivo and in vitro roles of ROCK2 in the regulation of adipogenesis and the development of obesity. We performed molecular, histological, and metabolic analyses in ROCK2^+/- and ROCK2^+/KD mouse models, the latter harboring an allele with a kinase-dead (KD) mutation. Both ROCK2^+/- and ROCK2^+/KD mouse models showed a lean body mass phenotype during aging, associated with increased amounts of beige cells in subcutaneous white adipose tissue (sWAT) and increased thermogenic gene expression in all

SUBMITTER: Wei L 

PROVIDER: S-EPMC6956740 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature