Aspergillus niger upregulated glycerolipid metabolism and ethanol utilization pathway under ethanol stress.

ABSTRACT: The knowledge of how Aspergillus niger responds to ethanol can lead to the design of strains with enhanced ethanol tolerance to be utilized in numerous industrial bioprocesses. However, the current understanding about the response mechanisms of A. niger toward ethanol stress remains quite limited. Here, we first applied a cell growth assay to test the ethanol tolerance of A. niger strain ES4, which was isolated from the wall near a chimney of an ethanol tank of a petroleum company, and found that it was capable of growing in 5% (v/v) ethanol to 30% of the ethanol-free control level. Subsequently, the metabolic responses of this strain toward ethanol were investigated using untargeted metabolomics, which revealed the elevated levels of triacylglycerol (TAG) in the extracellular components,

SUBMITTER: Vinayavekhin N 

PROVIDER: S-EPMC6957411 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature