Chitin deacetylases Cod4 and Cod7 are involved in polar growth of Aspergillus fumigatus.

ABSTRACT: Chitin is one of the key components of fungal cell wall, and chitin deacetylases (CDAs) have been found in fungi; however, their functions remain unknown. Aspergillus fumigatus is known to cause fatal invasive aspergillosis (IA) among immunocompromised patients with a high mortality rate. Although the A. fumigatus cell wall has long been taken as a unique target for drug development, its dynamic remodeling is complicated and not well understood. Seven putative CDAs are annotated in the A. fumigatus genome. In this study, we analyzed the function of the putative CDAs, Cod4 and Cod7, in A. fumigatus. Biochemical analysis of recombinant proteins showed that Cod4 preferentially deacetylated (GlcNAc)₄ and was less active on chitooligosaccharides with DP > 5, whereas Cod7 was unable t

SUBMITTER: Xie M 

PROVIDER: S-EPMC6957412 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature