Resolving kinetic intermediates during the regulated assembly and disassembly of fusion pores.

ABSTRACT: The opening of a fusion pore during exocytosis creates the first aqueous connection between the lumen of a vesicle and the extracellular space. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediate the formation of these dynamic structures, and their kinetic transitions are tightly regulated by accessory proteins at the synapse. Here, we utilize two single molecule approaches, nanodisc-based planar bilayer electrophysiology and single-molecule FRET, to address the relationship between SNARE complex assembly and rapid (micro-millisecond) fusion pore transitions, and to define the role of accessory proteins. Synaptotagmin (syt) 1, a major Ca²⁺-sensor for synaptic vesicle exocytosis, drove the formation of an intermediate: committed trans-SNARE com

SUBMITTER: Das D 

PROVIDER: S-EPMC6957489 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature