Annexin-V stabilizes membrane defects by inducing lipid phase transition.

ABSTRACT: Annexins are abundant cytoplasmic proteins, which bind to membranes that expose negatively charged phospholipids in a Ca²⁺-dependent manner. During cell injuries, the entry of extracellular Ca²⁺ activates the annexin membrane-binding ability, subsequently initiating membrane repair processes. However, the mechanistic action of annexins in membrane repair remains largely unknown. Here, we use high-speed atomic force microscopy (HS-AFM), fluorescence recovery after photobleaching (FRAP), confocal laser scanning microscopy (CLSM) and molecular dynamics simulations (MDSs) to analyze how annexin-V (A5) binds to phosphatidylserine (PS)-rich membranes leading to high Ca²⁺-concentrations at membrane, and then to changes in the dynamics and organization of lipids, e

SUBMITTER: Lin YC 

PROVIDER: S-EPMC6957514 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature