Effects of intravitreal injection of human CD34⁺ bone marrow stem cells in a murine model of diabetic retinopathy.

ABSTRACT: Human CD34 ⁺ stem cells are mobilized from bone marrow to sites of tissue ischemia and play an important role in tissue revascularization. This study used a murine model to test the hypothesis that intravitreal injection of human CD34 ⁺ stem cells harvested from bone marrow (BMSCs) can have protective effects in eyes with diabetic retinopathy. Streptozotocin-induced diabetic mice (C57BL/6J) were used as a model for diabetic retinopathy. Subcutaneous implantation of Alzet pump, loaded with Tacrolimus and Rapamycin, 5 days prior to intravitreal injection provided continuous systemic immunosuppression for the study duration to avoid rejection of human cells. Human CD34 ⁺ BMSCs were harvested from the mononuclear cell fraction of bone marrow from a healthy dono

SUBMITTER: Yazdanyar A 

PROVIDER: S-EPMC6957710 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature