Effects of prenatal hypoxia on fetal sheep heart development and proteomics analysis.

ABSTRACT: The present study aimed to investigate abnormal fetal cardiac pathological injury with exposure to a hypoxic environment during pregnancy. Pregnant hypoxia sheep models were prepared using artificial cabins. Sixteen pregnant sheep were randomly divided into three groups: normal group (NG), mild hypoxia group (MHG), and severe hypoxia group (SHG). The degree of SpO₂, SaO₂, breathing and heart rate, CO, and MDA were determined. HE staining, SEM, and TEM were used to evaluate the pathological changes of fetal myocardium. iTRAQ-based quantitative proteomics was employed to identify differentially expressed proteins. At day 30 and 90, the levels of SpO₂ and SaO₂ in SHG and MHG sheep met the diagnostic criteria. Ninety days after modeling, a dramatic r

SUBMITTER: Li H 

PROVIDER: S-EPMC6958198 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature