Expression of Long Non-Coding RNAs by Human Retinal Muller Glial Cells Infected with Clonal and Exotic Virulent Toxoplasma gondii.

ABSTRACT: Retinal infection with Toxoplasma gondii-ocular toxoplasmosis-is a common cause of vision impairment worldwide. Pathology combines parasite-induced retinal cell death and reactive intraocular inflammation. Müller glial cells, which represent the supporting cell population of the retina, are relatively susceptible to infection with T. gondii. We investigated expression of long non-coding RNAs (lncRNAs) with immunologic regulatory activity in Müller cells infected with virulent T. gondii strains-GT1 (haplogroup 1, type I) and GPHT (haplogroup 6). We first confirmed expression of 33 lncRNA in primary cell isolates. MIO-M1 human retinal Müller cell monolayers were infected with T. gondii tachyzoites (multiplicity of infection = 5) and harvested at 4, 12, 24, and 36 h post-infection, with infec

SUBMITTER: Rochet E 

PROVIDER: S-EPMC6958423 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature