Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells.

ABSTRACT:

Background

Long non-coding RNAs (lncRNAs) have been widely known to have an appreciable effect in physiology and pathology. In tooth regeneration, periodontal ligament stem cells (PDLSCs) are regarded as a key effector, whereas, how lncRNA acts in the osteogenic differentiation of PDLSCs have not been completely understood. This study aims to find out the relationship between lncRNA DANCR and the proliferation and osteogenic differentiation of PDLSCs.

Methods

Microarray was used to observe the different expression of lncRNAs in differentiated and undifferentiated PDLSCs. And then osteogenic-related lncRNA, DANCR was screened out. Its effects on proliferation and osteogenic differentiation was explored by constructing an overexpression and inhibition model. qRT-PCR was used t

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6958786 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature