Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia.

ABSTRACT: Many studies have shown that microRNAs (miRNAs) play vital roles during the spermatogenesis. However, little is known about the altered miRNA profiles of testicular tissues in nonobstructive azoospermia (NOA). Using microarray technology, the miRNA expression profiles of testicular biopsies from patients with NOA and of normal testicular tissues were determined. Bioinformatics analyses were conducted to predict the enriched biological processes and functions of identified miRNAs. The microarray data were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), the results of which were then validated with a larger sample size. Correlations between the miRNA expression levels and clinical characteristics were analyzed. Receiver operating characteristic (ROC) curv

SUBMITTER: Zhang HT 

PROVIDER: S-EPMC6958976 | biostudies-literature | 2020 Jan-Feb

REPOSITORIES: biostudies-literature